Human adenocarcinoma cells (HeLa line) fluorescence
These cells, derived from the adenocarcinoma of the cervix of a 31-year-old woman Henrietta Lacks in 1951, are probably the most commonly used cell line in the world. The cells circumvent the Hayflick limit, which makes them an ‘immortal’ line with the ability to continue the cell division after unlimited times. Using it, researchers developed vaccines against polio, human papillomavirus, discovered telomerase and did hundreds of investigations due to received knowledge. According to the Cell, it is expected that in 2010 more than 50 million tons of HeLa cells were replicated over previous decades. PubMed provides near 100.000 papers with ‘HeLa’ as the keyword.
Next dyes were used in the electronic immunofluorescence image: 📌Cy2 with anti-tubulin antibodies for tubulin (green); 📌Alexa Fluor 568 conjugated to phalloidin for the cytoskeletal F-actin network (orange-red); 📌Hoechst 33258 for DNA in the nuclei of cells (blue).
Source: National High Magnetic Field Laboratory, the Optical Microscopy Division